Sex Chromosomal Analysis of Spermatozoa from Infertile Men Using Fluorescence In Situ Hybridization

摘要

>Purpose: To confirm an association between male infertilityand chromosome aberrations of spermatozoa, wedemonstrated the frequency of numerical abnormalities ofspermatozoa from infertile men with abnormal semen parameterscompared with fertile controls.>Method: Sperm cells from 10 infertile patients wereinvestigated for disomy rates of sex chromosomes and chromosome18 and diploidy by fluorescence in situ hybridization (FISH).All patients showed oligoasthenozoospermia with spermcounts 3–20 × 10⁶/ml and motile rates 0–40%.>Results: Regarding XY disomy, a significantly higherfrequency was found in 8 of 10 patients as compared to normalfertile men. The disomy rates of chromosome 18, XX, YY,and diploidy rate were not increased.>Conclusions: There is an association between maleinfertility and embryo with aneuploidy of sex chromosomes.Counseling about possible genetic risks should be provided to theinfertile couples planning assisted reproduction treatment.