The europium chelate of 4,4,4-trifluoro-1-(2-thienyl)-1,3-butanedione (thenoyl-trifluoroacetone; TTA) is firmly bound to microorganisms. It fluoresces brightly at 613 nm with activation at 340 nm. Cells may be stained with 10−3m chelate in 50% ethyl alcohol, followed by washing with 50% ethyl alcohol. Equal or better stains are produced with 10−3m aqueous europium salt, water wash, and 10−2m aqueous TTA. A noncomplexing buffer should be used to maintain the pH at 6.5 to 6.8.
展开▼
机译：4,4,4-三氟-1-（2-噻吩基）-1,3-丁二酮（the酰基-三氟丙酮； TTA）的che螯合物牢固地与微生物结合。它在613 nm处发出明亮的荧光，并在340 nm处激活。可用在50％乙醇中的10 -3 螯合物对细胞染色，然后用50％乙醇洗涤。用10 -3 m euro盐水溶液，水洗和10 -2 m TTA水溶液可产生相同或更好的污点。应使用非复合缓冲液将pH值保持在6.5至6.8。
展开▼
