Posthypoxic behavioral impairment and mortality of Drosophila melanogaster are associated with high temperatures enhanced predeath activity and oxidative stress

摘要

Schematic illustration of the hypoxia protocol in D. melanogaster. a The frequently used wild-type D. melanogaster strains Canton-S and Oregon-R were subjected to different durations (1–6 h) of severe hypoxia (<0.3% O2) followed by 120 h of reperfusion. Behavioral testing (negative geotaxis and DAM assays), mortality rate assessment, and gene and protein analyses were performed in the posthypoxic reperfusion phase. b Death rates of Canton-S D.m. and c Oregon-R D.m. after 1 to 6 h of severe hypoxia (<0.3% O2) followed by 120 h of reperfusion. d Nonlinear regression of the hypoxia duration-dependent death rates of Canton-S D.m. and Oregon-R D.m. The data are shown as the mean ± SEM from 4 independent experiments including a total of 240 male flies per genotype for each hypoxia period.