Streamlined human antibody generation and optimization by exploiting designed immunoglobulin loci in a B cell line

摘要

The diversification mechanism of avian immunoglobulin genes and the principle of the ADLib system. a Diversification of avian immunoglobulin genes by GC. Partial sequences of the pseudogenes are transferred to the functional V region by homologous recombination. GC is a unidirectional event in which the functional V region is altered while the pseudogenes remain unchanged. b Principle of the ADLib system. DT40 cells are cultured in the presence of TSA to generate diversified cell-based mAb libraries. Antigen-specific clones can be isolated by, for example, antigen-conjugated magnetic beads. The isolated cells are expanded, and the antigen-specific mAbs are recovered in culture supernatants. The process, from magnetic bead selection to the recovery of the mAbs, can be completed in ~10 days