Erlotinib protests against LPS-induced parthanatos through inhibiting macrophage surface TLR4 expression

摘要

Wild-type C57BL/6 mice were primed with 100 mg kg–1 of erlotinib gavage administration or 3-AB before LPS treatment as described in methods. a After LPS stimulation, survival rate of mice was detected every 6 h for 72 h. LPS + erlotinib group (n = 20) and LPS + 3-AB group (n = 20)’s life span was significantly increased compared to LPS group (n = 20, P < 0.05). b, c After 24 h LPS treatment, peritoneal macrophages were collected and identified with F4/80. Cell death was analyzed by flow cytometry. d, e RAW264.7 cells were treated with LPS for 24 h in the presence or absence of pretreatment of erlotinib or 3-AB for 30 min followed by flow cytometry analysis of cell death. Error bars represent SD (n = 3). *P < 0.05 as compared with Sham; †P < 0.05 as compared with the time-matched LPS group.