HOTAIRM1 regulates neuronal differentiation by modulating NEUROGENIN 2 and the downstream neurogenic cascade

摘要

a qRT-PCR analysis of HOTAIRM1 and NEUROG2 expression along differentiation of iPSCs into ventral spinal cord lineages. Differentiation days are reported on the x-axis. Expression levels are relative to ATP5O and expression peaks are set as 1. N = 1, 2 or 3, depending on the sample. **P ≤ 0.0021, ***P ≤ 0.0002. b qRT-PCR analysis of mRNAs coding for MYCN (proliferative marker), VGF (neuropeptide precursor) and LAMC1 (neurite-promoting factor) along neuronal differentiation of SH-SY5Y cells. Days of RA treatment are reported on the x-axis. Expression levels are relative to GAPDH, expression peaks are set as 1. N = 3, *P ≤ 0.05, **P ≤ 0.01, ***P ≤ 0.001. c qRT-PCR analysis of HOTAIRM1 and NEUROG2 expression along neuronal differentiation of SH-SY5Y cells. Broken axis-histogram allows to appreciate low values. Details as in (b). N = 2 or 3, depending on the target. *P ≤ 0.05, **P ≤ 0.01, ***P ≤ 0.001. d Immunoblot analysis of NEUROG2 along neuronal differentiation of SH-SY5Y cells. Days of RA treatment are reported below each lane. In the histogram, NEUROG2 levels are relative to GAPDH. N = 3, **P ≤ 0.01.