LncRNA SNHG15 regulates EGFR-TKI acquired resistance in lung adenocarcinoma through sponging miR-451 to upregulate MDR-1

摘要

a IC50 of A549, H1975, A549/GR, and H1975/GR was detected. b qRT-PCR was conducted to investigate the expression of signaling pathway receptors (NOTCH-1, NOTCH-2, NOTCH-3, and NOTCH-4) and ligands (Jagged-1, Jagged-2, and Delta-1) in human lung adenocarcinoma cells (A549 and H1975) and their relevant drug-resistant cell lines (A549/GR and H1975/GR). c The protein level of NOTCH-1was tested via western blot. d The alteration of SNHG15 expression caused by sh-NOTCH-1 was tested in A549/GR and H1975/GR via qRT-PCR. e qRT-PCR was used to test the interference efficiency of SNHG15. f–i The proliferation, apoptosis, and cell cycle of A549/GR and H1975/GR were investigated by colony formation assay, EdU assay and flow cytometry analysis. Data obtained from three replications were shown as mean ± S.D. *P < 0.05, **P < 0.01 indicated that differences were statistically significant.