Relationships between UBE3A and SNORD116 expression and features of autism in chromosome 15 imprinting disorders

摘要

Determining stability of expression for 7 internal control genes using the geNorm approach in peripheral blood mononuclear cells (PBMCs) of 44 individuals affected with PWS (n = 21) and typically developing controls (n = 23). A Average expression stability M values, with least to most stable ordered in the left to right direction on the x-axis. SDHA, ATP5N and E1F4A2 were the most stably expressed genes from the panel tested. B Variation in average gene expression stability with sequential addition of each internal control gene to the equation (for calculation of the V score normalisation factor). In A, the least stably expressed genes are shown on the left side of the x-axis and the most stably expressed genes or combinations of genes are shown on the right side (i.e., ATP5B and SDHA). Note: All internal control primer/probe mixes were obtained from PrimerDesign (PerfectProbe ge-PP-12-hu kit) and used at a concentration of 2 μM.