CPEB3 inhibits epithelial-mesenchymal transition by disrupting the crosstalk between colorectal cancer cells and tumor-associated macrophages via IL-6R/STAT3 signaling

摘要

Decreased CPEB3 in human CRC correlates with low CD86+ TAM content and high CD163+ TAM content (a) The expression of CPEB3 and CD86 in 82 pairs of CRC tissues and adjacent non-tumor tissues was detected using qRT-PCR. Correlation between CPEB3 and CD86 or CD163 expression levels in 82 colorectal cancer tissues; error bars, SEM. (b) The protein expression of CPEB3, CD68, CD86, and CD163 in a human colorectal cancer tissue array was detected by IHC staining. Representative photos are shown (400× magnification). The number of CD68+, CD86+ and CD163+ cells per high-power field in tissues from colorectal cancer patients with different levels of CPEB3 expression; error bars, SEM. (c) Schema for an in vitro model of stably transfected CRC cells co-cultured with TAMs. (d) Flow cytometry was used to explore the surface expression of CD86 and CD163 in SW480-Ctrl/CPEB3 and HCT116-Ctrl/CPEB3 cells; error bars, SEM. (e) Flow cytometry was used to explore the surface expression of CD86 and CD163 in LoVo-shCtrl/shCPEB3 and RKO-shCtrl/shCPEB3 cells; error bars, SEM. (f) We measured the expression of the respective inflammatory cytokines in cell culture supernatants of TAMs co-cultured HCT116-Ctrl/CPEB3 cells using ProcartaPlex combinable panels; error bars, SEM. (g) We measured the expression of the respective inflammatory cytokines in cell culture supernatants of TAM-co-cultured LoVo-shCtrl/shCPEB3 cells using ProcartaPlex combinable panels; error bars, SEM; ns, not significant; * P < 0.05; ** P < 0.01; *** P < 0.001; **** P < 0.0001