Grapevine VlbZIP30 improves drought resistance by directly activating VvNAC17 and promoting lignin biosynthesis through the regulation of three peroxidase genes

摘要

aAgrobacterium-mediated transformation of Thompson Seedless. 1 Callus induction from flower buds. 2 Proembryonal masses (PEMs) used for transformation. 3 Coculturing of PEM and Agrobacterium EHA105 harboring the CaMV35S-3×Flag-VlbZIP30 plasmid. 4 Kanamycin-resistant embryogenic callus. 5 Kanamycin-resistant somatic embryos. 6 Somatic embryo germination and plantlet formation. 7 Three-month-old WT and six transgenic plantlets with higher expression levels of VlbZIP30 than the WT. b Identification of T-DNA insertion in 47 transgenic lines. The numbers indicate the transgenic lines. c Schematic diagram of the recombinant construct (CaMV35S-3×Flag-VlbZIP30). d Quantitative real-time (qRT)-PCR analysis of VlbZIP30 transcript abundance in 12 transgenic lines. The grapevine VvActin1 gene was used as an internal control. Values are means ± SEs (n = 3). Asterisks indicate statistical significance (*0.01 < P < 0.05, **P < 0.01, Student’s t test) between the WT and transgenic lines