Dibutyl phthalate promotes juvenile Sertoli cell proliferation by decreasing the levels of the E3 ubiquitin ligase Pellino 2

摘要

The effect of dibutyl phthalate (DBP) on Sertoli cell (SC) proliferation. a The effect of DBP on the number of Sertoli cells per testis in mice after prenatal exposure to DBP. Testicular sections were collected from pups 22 days after mice were exposed in utero (GD12.5 - birth) to corn oil or DBP doses of 50, 250 or 500 mg/kg/day. Immunohistochemical staining for SOX9 was performed (scale bar 50 μm). Arrows represent the expression of SOX9 in the testes of DBP-treated and control male pups. b The ratio of SOX9-positive cell was detected by ImageJ (n = 6). The results are expressed as the means ± SEM. * p < 0.05; ** p < 0.01, compared with control