Infected B. glabrata vector snails were stimulated under light to release (“shed”) 104–105S. mansoni infective larvae (cercariae) per week. Cercariae were mechanically converted to post-infective larvae (schistosomula or somules) using a 20-gauge double-headed syringe needle. After washing to remove cercarial tails, somules were suspended in a paddle-stirred reservoir. Somules were then dispensed into 96-well u-bottom clear plates at 40 units/well/200 µL Basch medium. Compounds were added using a 96-channel pin tool. Plates were maintained at 5% CO2 and 37 °C. At specified time points, a six-axis robotic arm transferred the plates to the high-content imager.
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