Fluorescent artificial receptor-based membrane assay (FARMA) for spatiotemporally resolved monitoring of biomembrane permeability

摘要

a Encapsulation of membrane-impermeable fluorescent artificial receptors (FARs) into liposomes spatially separates the FARs from the subsequently added analyte. Upon analyte permeation through the membrane into the liposome, and subsequent rapid analyte complexation by FAR, a readily observable change in the fluorescence intensity of the FAR can be observed (typically emission quenching). b Expected emission-readout for a permeable vs. an impermeable analyte. c Structures of the synthetic host CB8 and dyes (D1–D3), from which FAR-1, FAR-2, and FAR-3 were assembled.