The pro-survival Bcl-2 family member A1 delays spontaneous and FAS ligand-induced apoptosis of activated neutrophils

摘要

Survival analysis of neutrophils from mice with the indicated genotypes cultured in a simple medium (no added cytokines), b after stimulation with 10 ng/mL GM-CSF plus 10 ng/mL LPS, c after treatment with Fc-FASL (0.6 ng/mL), and d after stimulation with LPS plus GM-CSF (10 ng/mL each) and Fc-FASL (0.6 ng/mL). e FASL-specific apoptosis when compared to survival of cells stimulated with LPS plus GM-CSF. Data are from five combined experiments (WT n = 9, A1–/–n = 9, Mcl-1+/–n = 6, Mcl-1+/–A1–/–n = 7, Bid–/–n = 7, and Bid–/–A1–/–n = 7 mice). Statistical significance (*P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001) was determined using Student’s t-test at each timepoint compared to WT (*) or A1–/– (†).