Hypoxia-induced shift in the phenotype of proteasome from 26S toward immunoproteasome triggers loss of immunoprivilege of mesenchymal stem cells

摘要

Human bone marrow-derived MSCs were incubated in hypoxia chamber for 24 h. a Protein levels of PSMD11, PSMD4 (Rpn10), PSMB6 (β1), PSMB7 (β2), and PSMB5 (β5) as measured by Western blot showed a significant decrease in hypoxic MSCs compared to normoxic cells; n = 3. b, c Immunofluorescence images exhibited a significant decrease in the expression of PSMD4 (Rpn10), PSMD11, PSMB6 (β1), PSMB7 (β2) and PSMB5 (β5) in hypoxic MSCs compared to normoxic cells; n = 4. *p < 0.05 compared to normoxic MSCs. Each experiment was repeated 3–4 times.