Glutathione S-transferases P1 protects breast cancer cell from adriamycin-induced cell death through promoting autophagy

摘要

GSTP1 contributes to the resistance of MCF-7 to ADR. a Immunoblot analysis of GSTP1 protein in MCF-7 and MCF-7/ADR cells. b, c MCF-7 cells transfected with pcDNA (2 μg/mL) or Flag-GSTP1 (2 μg/mL) were treated with an indicated dose of ADR for 48 h (b). MCF-7/ADR cells transfected with Scramble-shRNA (2 μg/mL) or GSTP1-shRNA (2 μg/mL) plasmid were treated with 0, 0.1, 1, 10, 100 μM ADR for 48 h (c). Cell viabilities were detected by MTT assay. d MCF-7 transfected with pcDNA (2 μg/mL) or Flag-GSTP1 (2 μg/mL) plasmids and MCF-7/ADR cells transfected with Scramble-shRNA (2 μg/mL) or GSTP1-shRNA (2 μg/mL) plasmid were treated with 10 μM ADR for 48 h, and the apoptotic cells were assessed by annexin V-FITC/PI staining. e MCF-7 cells were transfected with either pcDNA (2 μg/mL), wild type GSTP1 (WT) (2 μg/mL), or catalytically inactive mutant GSTP1 (Y7F) (2 μg/mL), and 24 h after transfection cells were treated with 10 μM ADR for 48 h. Cell viability was detected using MTT assay. f MCF-7/ADR cells were pre-treated with NBDHEX for 2 h followed by treatment with ADR for 48 h, and then the viability of cells was detected by MTT assay. Data of at least 3 independent experiments performed in duplicates is presented as mean ± SEM. *P < 0.01, **P < 0.01, ***P < 0.001 compared with control. ##P < 0.01, ###P < 0.001 compared with control plasmid transfected cells