Hexokinase inhibition using D-Mannoheptulose enhances oncolytic newcastle disease virus-mediated killing of breast cancer cells

摘要

MH and oncolytic AMHA1 NDV are cytotoxic against human AMJ13 and MCF-7 breast cancer cells, but not cytotoxic to normal embryonic REF cells. The cells were treated with (a–c) D-Mannoheptulose (MH) (13.125, 26.25, 52.5, 105, 210, 840, and 1680 μg/ml) or (d–f) NDV (MOI 0.1, 0.2, 0.4, 0.8, 1.6, 3.2, 6.4, and 12.8) for 72 h. cytotoxicity was investigated using MTT assay and showed that IC50 values of MH (486.9 µg REF, 124.7 µg AMJ13, and 122.6 µg MCF7) and IC50 values for NDV MOI (57.5 REF, 1.648 AMJ13 and 1.561 MCF-7). All data shown are mean ± SEM from three independent experiments