Mechanical stimulation of human dermal fibroblasts regulates pro-inflammatory cytokines: potential insight into soft tissue manual therapies

摘要

a Schematic representation of cell culturing method designed to allow matched comparison between unstrained primary human dermal fibroblasts (Control) and primary human dermal fibroblasts subjected to cyclic short-duration strain (CSDS). All conditioned media was collected simultaneously 24 or 96 h after the onset of the CSDS strain profile. b Schematic representation of CSDS profile used for studies in Fig. 1 and ​and2.2. c ELISA for interleukin (IL)-6 collected from primary dermal fibroblasts 96 h following CSDS compared to time-matched unstrained controls. Data are mean ± SEM normalized to Control; n = 3 per condition. * indicates p < 0.05 against Control by paired t-test