LIM domain proteins Pinch1/2 regulate chondrogenesis and bone mass in mice

摘要

Pinch1Prx1; Pinch2−/− (dKO), but not single mutant (Pinch1Prx1 or Pinch2−/−), mice display dwarfism. a Representative pictures of 6-week-old male control (Prx1-Cre), Pinch1Prx1, Pinch2−/−, and dKO mice. Scale bar, 1 cm. b Growth curve. The results are expressed as the mean ± standard deviation (s.d.). Student’s t test, *P < 0.05; **P < 0.01; ***P < 0.001. N = 5 control, Pinch1Prx1 and Pinch2−/− mice; N = 7 dKO mice. c Survival curve. N = 7 mice per group. Alcian blue-alizarin red double staining of whole-mount skeletons (d), rib cages and sterna (e), and calvaria (f) of P0 control, Pinch2−/−, Pinch1Prx1, and dKO mice. Scale bar, 5 mm. The red arrow indicates the bifurcation of the sternum in dKO mice. g Alcian blue/hematoxylin/Orange G staining of humeral sections from E18.5 and P0 control and dKO mice. Scale bar, 200 mm. h Hematoxylin and eosin (H/E) staining of humeral sections from E18.5 and P0 control and dKO mice. Scale bar, 100 μm. i–k Alcian blue-alizarin red staining of tibial sections from P17 and 6-week-old male control, Pinch2−/−, Pinch1Prx1, and dKO mice (i). Quantification of the lengths of the proliferative zone (PZ) (j) and hypertrophic zone (HZ) (k). N = 5 mice per group. Student’s t test. The results are expressed as the mean ± s.d. ***P < 0.001. Scale bar, 400 μm (top and middle) or 80 μm (bottom)