Establishment of a PEG-mediated protoplast transformation system based on DNA and CRISPR/Cas9 ribonucleoprotein complexes for banana

摘要

Detection of GFP fluorescence in transformed protoplasts by fluorescence microscope. pUbi-GFP plasmid was used for protoplast transformation under different transformation conditions: 40% PEG and 20 min incubation (a), 40% PEG and 30 min incubation (b), 50% PEG and 20 min incubation (c), 50% PEG and 30 min incubation (d). The protoplasts from 50% PEG with 30 min mixing were examined by flow cytometry (e). All pictures were taken after 5 days incubation in darkness. Scale bars are 75 μm