Development and clinical application of a novel CRISPR-Cas12a based assay for the detection of African swine fever virus

摘要

Schematic of the CRISPR-Cas12a based assay for ASFV detection. Viral nucleic acids from different sample types were extracted, and the p72 target region amplified using RPA at 39 °C for 20 min. The amplicon was then mixed with Cas12a/crRNA, and a ternary complex formed when the target region was present. Finally binding to the target site resulted in the cleavage of the reporter ssDNA producing a signal. The image shown in Fig. 1 was made by us and belongs to us