Serum keratin 19 (CYFRA21-1) links ductular reaction with portal hypertension and outcome of various advanced liver diseases

摘要

Hepatic expression of genes related to ductular reaction in patients with chronic liver disease (cohort i). Fifty-seven livers from patients with various stages of liver fibrosis (fibrosis stages F0–F4) were analyzed (cohort i). a–c Relative hepatic expression of keratins 19, 7, and 23 (K19/K7/K23), compared to RPLP0. Average K7/19/23 expression in control subjects was arbitrarily set as 1 and all other levels represent a ratio. d, e Spearman’s correlation coefficient quantifies the relationship between the hepatic expression of K19 mRNA and K7 (d) or K23 (e). f K19 protein levels were determined by immunoblotting and tubulin was used as a loading control. The relative band intensity (lower panel) was quantified using ImageJ. *P < .05, **P < .01, ***P < .001, ****P < .0001