Comparative gene expression profiling of mouse ovaries upon stimulation with natural equine chorionic gonadotropin (N-eCG) and tethered recombinant-eCG (R-eCG)

摘要

A schematic representation of tethered R-eCG. The wild-type protein with N- and O-linked oligosaccharide sites on eCG are shown. The circle “N” denotes an N-linked oligosaccharide. eCG α-subunit consists of 96-amino acids identical to those of LH, FSH, and TSH. eCG α-subunit contains two N-linked oligosaccharides at amino acid positions 56 and 82. eCG β-subunit contains one N-linked oligosaccharide site at position 13 and up to 12 O-linked oligosaccharide sites in the C-terminal region. Thus, tethered eCGβα gene was linked to C-terminal region of β-subunit with α-subunit without signal sequence. Natural eCG model is displayed as dimeric N-eCGα+β