Clinical effect and antiviral mechanism of T-705 in treating severe fever with thrombocytopenia syndrome

摘要

Infectivity and mutation analysis of SFTSV in four passages of cell culture supernatant from cells treated with different concentrations of T-705. SFTSV strain HNXY2017-66 was serial passaged in T-705 or vehicle-treated Vero cells for four passages (P1–P4). The cell culture supernatant was collected for the NGS and mutation analysis of the SFTSV genome. Total mutation rates (a), ratios of transitions/transversions (c), transition mutation rates (d), and percentages of different transition mutations (e) were calculated. Viral titer and viral genome copy number in the supernatant were also measured, and relative viral infectivity (viral titer/viral genome copy number) is shown (b)