Adult Stress Promotes Purinergic Signaling to Induce Visceral Pain in Rats with Neonatal Maternal Deprivation

摘要

Co-expression of β2-ARs with P2X3Rs in DRG Neurons and Enhancement of ATP-induced Ca2+ Signaling by NE incubation. A β2-ARs and P2X3Rs are co-expressed in colorectal-related DRG neurons (red, β2-AR-positive cells; green, P2X3R-positive cells; arrows, co-labeled cells; scale bar, 50 μm). B Analysis showing that β2-ARs are co-expressed with P2X3R-positive DRG neurons, and P2X3Rs are also co-expressed with β2-AR-positive DRG neurons (n = 3). C An example from a cell showing responses to ATP (20 μmol/L) before and after incubation with norepinephrine (NE, 20 μmol/L) for 5 min. NE significantly enhanced the ATP-induced Ca2+ signals compared with extracellular solution (EXTRA) (***P < 0.001; n = 33, paired t test).