Distinct role of 5′UTR sequences in dendritic trafficking of BDNF mRNA: additional mechanisms for the BDNF splice variants spatial code

摘要

Trafficking of GFP, exon 1-GFP, and exon 4-GFP transcripts in hippocampal neurons. a Representative image of an in situ hybridization against the GFP coding sequence on one hippocampal pyramidal neuron transfected with pEGFP-N1 plasmid before (upper panel) and after straightening (central panel). Bottom panels represent two control in situ hybridization on pEGFP-N1 transfected neurons using GFP coding sequence sense probe (left) and on untransfected neurons using antisense probe. Scale bar: 10 μm. b–d Densitometric analysis on in situ hybridization of apical dendrites of neurons transfected with GFP (b), exon 1-GFP (c), and exon 4-GFP (d) untreated (gray boxes) or treated with 10 mM KCl for 3 h (white boxes). Below each graph, representative straightened neurons labeled by in situ hybridization are reported. Lines in graphs C and D represent the plot of the median gray value of GFP densitometric analysis untreated (solid line) and after KCl stimulation (dashed line). Scale bar: 10 μm