Mechanistic insights into the role of the chemokine CCL2/CCR2 axis in dorsal root ganglia to peripheral inflammation and pain hypersensitivity

摘要

The chemokine CCL2 induces a CCR2-dependent pain hypersensitivity in the formalin model of persistent pain. Intrathecal administration of vehicle (DMSO 6%), 3 μg/kg CCL2 or 45 μg/kg INCB3344 with or without CCL2 is performed 1h prior to intraplantar formaldehyde (1%). The cumulative nociceptive response, expressed as mean area under the curve (A.U.C.), is measured during the inflammatory phase. Data correspond to mean ± SEM (n = 8). Asterisks denote statistical significance (*, #P < 0.05; ANOVA followed by Bonferroni’s multiple comparison test)