Comparison of the proteomes in sera between healthy Thoroughbreds andThoroughbreds with respiratory disease associated with transport using massspectrometry-based proteomics

摘要

In the past decade, mass spectrometry has become an important technology for proteinidentification. Recent developments in mass spectrometry allow a large number ofidentifications in samples; therefore, mass-spectrometry-based techniques have beenapplied to the discovery of biomarkers. Here, we conducted a proteomic study to comparethe proteomes in sera between healthy Thoroughbreds and Thoroughbreds with respiratorydisease associated with transport (RDT). We found that four proteins, apolipoprotein F,lipopolysaccharide binding protein, lysozyme and protein S100-A8, were upregulated, whilekeratin 1 was downregulated in the RDT group. It is assumed that inflammation and immuneresponse are involved in the changes of these proteins. The findings suggested that theseproteins are potentially useful for elucidating the mechanism of development of RDT.