Transcriptomics-Based Approach Identifies Spinosad-Associated Targets in the Colorado Potato Beetle

摘要

The Colorado potato beetle Leptinotarsa decemlineata is a potato pest that can cause substantial damages to potato crops worldwide. Multiple approaches have been leveraged to control this pest including the use of a variety of insecticides. Resistance to different insecticides aimed at controlling this insect has been reported and much work has been conducted in recent years to elucidate the underlying molecular changes associated with insecticide resistance in L. decemlineata. However, information is sparse regarding the molecular impact associated with spinosad treatment in this insect pest. The current study thus explores transcriptional changes associated with spinosad response in L. decemlineata exposed to this compound using high-throughput sequencing. Results presented show multiple transcripts of interest that exhibit differential expression in spinosad-treated L. decemlineata and provide a preliminary footprint of transcripts affected by this insecticide in this potato pest. Select targets identified in this signature should be further explored in follow-up studies to better characterize their contribution, if any, in the process of spinosad resistance.