The transcription factor SlHY5 regulates the ripening of tomato fruit at both the transcriptional and translational levels

摘要

a Genotype of mutations in the SlHY5 locus generated by the CRISPR/Cas9 genome editing system. Two target sequences were designed to specifically target the second and third exons with a 214-bp interval. The red and blue letters indicate the sequences of targets 1 and 2, respectively. The protospacer adjacent motif (PAM) is indicated. The mutations in the transgenic plants were confirmed by sequencing the genomic regions flanking the target sites. The sequences of the wild-type (WT) plants and three homozygous mutant lines (Slhy5-2, Slhy5-13, and Slhy5-29) are shown. b Transcript levels of SlHY5 in wild-type and Slhy5 mutant fruits determined by quantitative real-time PCR. c SlHY5 protein levels in wild-type and Slhy5 mutant fruits determined by western blotting. The histone H3 protein levels were used as a loading control. d Ripening phenotype of Slhy5 mutants. Wild-type and Slhy5 mutant fruits at 36, 39, 42, and 45 days post anthesis (DPA) are shown