A small natural molecule CADPE kills residual colorectal cancer cells by inhibiting key transcription factors and translation initiation factors

摘要

a Apoptosis rates of CRC cells were measured by flow cytometry after treating with CADPE (25 μM) for 48 and 72 h (n = 3). b Expression levels of anti-apoptotic proteins in normal colon CCD-18Co and CRC cells and effect of CADPE (25 μM) on the expression levels of above anti-apoptotic proteins in CRC cells after the treatment of 48 h through immunoblot analysis. c HCT-15 and SW620 cells treated with CADPE (12.5 μM) for 24 h were subjected to immunocytological analysis. Bar: 30 μm. d Mitochondrial membrane potential (MMP) in CRC cells was altered after the treatment of CADPE (12.5 μM) for 24 and 48 h (n = 4). Data are presented as the mean ± SD, ***p < 0.001 (vs. CON) by one-way ANOVA followed by Tukey’s test.