Application of fetal cell-free DNA enrichment in non-invasive prenatal screening: experience from a single center in Eastern China

摘要

Sequencing cell-free DNA (cfDNA) from the peripheral blood of pregnant women has been successfully applied in prenatal screening for fetal chromosomal aneuploidies, known as non-invasive prenatal screening (NIPS).[1,2] With large-scale clinical practices, it is recognized that the accuracy and reliability of this method largely relies on the proportion of fetal cfDNA presenting in total cfDNA from maternal plasma, known as the fetal fraction.[3]