The DNA-helicase HELLS drives ALK

摘要

A Western blot shows HELLS expression in TLBR-2 HELLSKD cells after 48 h of doxycycline (DOX) induction. GAPDH was used as housekeeping gene. B qRT-PCR analysis of HELLS expression in TLBR-2 HELLSKD cells after 48 h of doxycycline induction. The values represent mean ± SEM (n = 3) *p < 0.05; **p < 0.01. C The heatmap depicts hierarchical clustering based on the 728 differentially expressed genes, whose read counts are Z-score normalized. Unsupervised hierarchical clustering was performed between DOX and CTRL samples (as indicated by the colored bar on columns) with a complete linkage method. Color intensity for each gene shows Z-score values ranging from red for upregulation and green for downregulation D Most significant enriched pathways (adjusted p-value<0.05) are represented showing the number of DE genes mapped in each considered pathway. E The heatmap depicts validated significantly downregulated genes. Green color bar shows fold difference on Log2 scale calculated between DOX and CTRL samples. Darker green represents the most downregulated genes. Genes in red were selected for further validations. F Immunofluorescence images of TLBR-2 HELLSKD cells and MAC2A HELLSKD cells after 48 h of doxycycline (DOX) induction. Cells were stained with DAPI, F-actin, and β-tubulin antibodies. The white scale bar represents 10 μm.