Design and Microfabrication of An On-Chip Oocyte MaturationSystem for Reduction of Apoptosis

摘要

In customary assisted reproductive technology (ART), oocyte culture occurs in static micro drops of Petridishes with vast media volume; while, the in vivo condition is dynamic. In this study, we aimed to improve the maturationefficiency of mammalian oocytes by designing an optimal microchamber array to obtain the integration of oocytetrapping and maturation within a microfluidic device and evaluate the role of microfluidic culture condition in lipidperoxidation level of the culture medium, in vitro matured oocytes apoptosis, and its comparison with the conventionalstatic system.