Interaction analysis of statistically enriched mutations identified in Cameroon recombinant subtype CRF02_AG that can influence the development of Dolutegravir drug resistance mutations

摘要

HIV-1 Integrase phylogenetic analysis inferred by ML. The Phylogenetic tree inferred in MEGA contains (n = 20) patient derived sequences in red box and (n = 287) online database sequence indicated in black box. HIV-1 reference sequences were acquired from the HIV-1 LANL database, using the 2010 data set. An ML tree was constructed using Mega version 7.0, with the Kimura 2 parameter. The alignment was based on HXB2 position 4351–5069, of approximately 700 bp in length. The percentage of replicate trees in which the associated taxa clustered together in the bootstrap test (1000 replicates) are shown next to the branches. The bootstrap values are above 70 indicating significant support for the branches simulated. The reference sequences are the unboxed sequences. All of the sequence’s clusters with HIV-1 subtype CRF02_AG. LANL, Los Alamos National Laboratory; MEGA, Molecular Evolutionary Genetics Analysis; ML, maximum likelihood