Development of TaqMan-based real-time RT-PCR assay based on

摘要

a The detection limit of qRT-PCR assay based on cRNA copy number. Amplification plot of 10-fold serial diluted cRNA ranging from 1.74 × 1011 to 1.74 × 104 copies/μL. b Standard curve of qRT-PCR assay. Ten-fold dilutions of control cRNA were assessed with the qRT-PCR assay. Log copies per μL and quantitation cycle (Cq) are plotted on the x-axis and y-axis, respectively