MicrobeAnnotator: a user-friendly comprehensive functional annotation pipeline for microbial genomes

摘要

Graphical summary of the MicrobeAnnotator pipeline. Starting from a set of protein sequences, MicrobeAnnotator iteratively searches against 2 or 4 databases depending on the mode used (standard or light). Proteins without a KO identifier or match are searched against the next database. Otherwise, its metadata (best match, product, KO identifier, the taxonomy of best hit, GO numbers, and Pfam and InterPro accessions) are stored. Finally, KO identifiers are extracted, and module completeness is calculated using the custom MicrobeAnnotator database. The results are compiled in a single matrix-like module completeness table and summary plots for all genomes combined