Genome Report: Whole Genome Sequence and Annotation of the Parasitoid Jewel Wasp Nasonia giraulti Laboratory Strain RV2Xu

摘要

Jewel wasps in the genus of are parasitoids with haplodiploidy sex determination, rapid development and are easy to culture in the laboratory. They are excellent models for insect genetics, genomics, epigenetics, development, and evolution. ( ) and ( ) are closely-related species that can be intercrossed, particularly after removal of the intracellular bacterium , which serve as a powerful tool to map and positionally clone morphological, behavioral, expression and methylation phenotypes. The reference genome was assembled using Sanger, PacBio and Nanopore approaches and annotated with extensive RNA-seq data. In contrast, genome is only available through low coverage resequencing. Therefore, assembly is in urgent need to advance this system. In this study, we report a high-quality assembly using 10X Genomics linked-reads with 670X sequencing depth. The current assembly has a genome size of 259,040,977 bp in 3,160 scaffolds with 38.05% G-C and a 98.6% BUSCO completeness score. 97% of the RNA reads are perfectly aligned to the genome, indicating high quality in contiguity and completeness. A total of 14,777 genes are annotated in the genome, and 72% of the annotated genes have a one-to-one ortholog in the genome. We reported 5 million SNPs which will facility mapping and population genomic studies in . In addition, 42 -specific genes were identified by comparing with genome and annotation. This is the first assembly for this important species in the model system, providing a useful new genomic toolkit.