Characterization of multicellular breast tumor spheroids using image data-driven biophysical mathematical modeling

摘要

Schematic of MCTS generation and data acquisition protocol. MCTS are generated using ultra-low attachment microplates which are cultivated for 4 days. Once formed, MCTS are embedded into a collagen invasion matrix containing fluorescent microspheres to track extracellular matrix deformation. Time-lapse microscopy imaging of MCTS systems occurred over a 72-h time span. Images were tiled, stitched and rigidly registered before estimating cell number and X and Y deformation for the model.