Early urinary protein changes during tumor formation in a NuTu-19 tail vein injection rat model

摘要

Workflow for the early detection of tumor-forming and non-tumor-forming processes. ( ) Experimental design of the NuTu-19 tail vein injection rat model. In the tumor group, urine samples were collected on days 0, 12, 27, 39 and 52 after tail vein injection of NuTu-19 cells. Lung tissues were harvested on days 35, 45, 52 and 60. In the control group, all lung tissues were harvested on day 60. ( ) Urine sample preparation and data analysis for tumor-forming and non-tumor-forming rats. The urinary proteomes of the two groups were identified using liquid chromatography coupled with tandem mass spectrometry (LC–MS/MS) analysis. Then, parallel reaction monitoring (PRM) targeted proteomic quantitative analysis was used to validate candidate biomarkers for the early detection of tumor-forming and non-tumor-forming processes.