A novel tracer for in vivo optical imaging of fatty acid metabolism in the heart and brown adipose tissue

摘要

Chemical synthesis and characterization of AlexaFFA. ( ) Schematic of the coupling reaction between Click-IT™ Alexa Fluor 647 DIBO Alkyne and 15-azidopentadecanoic acid producing two regioisomers. ( ) LC–MS (UV detection 254 nm) of Alexa Fluor 647 DIBO Alkyne (red line) and the coupling products (blue line) showing a doublet with an ionized mass of 1,444 m/z (regioisomer 1,2) and a by-product with an ionized mass of 1685 m/z. ( ) Mass spectrometry of the extracted coupling products (m/z = 1,444 ) showing closely eluting but distinct species.