Thy-1 depletion and integrin β3 upregulation-mediated PI3K-Akt-mTOR pathway activation inhibits lung fibroblast autophagy in lipopolysaccharide-induced pulmonary fibrosis

摘要

LPS-induced autophagy inhibition is accompanied with Thy-1 downregulation and integrin β3 upregulation in LPS-induced pulmonary fibrosis. The severity of pulmonary fibrosis was determined by hematoxylin-eosin (H&E) staining; collagen deposition was revealed by MASSON staining (magnification, ×200) ( ). The expression of α-SMA in lung tissues was also measured by Western blot ( ). Collagen deposition was measured by hydroxyproline content and collagen level assessments ( ). Representative Western blot images showing expression of Thy-1, integrin β3, LC3 and P62 in the lung tissue ( ). Values are mean ± SD (  = 6). *  P