The MultiBac Baculovirus/Insect Cell Expression Vector System for Producing Complex Protein Biologics

摘要

The MultiBac baculovirus/insect cell expression system. The MultiBac system is shown in a schematic view ( ). MultiBac consist of an engineered baculovirus optimized for protein complex production. This MultiBac baculoviral genome exists as a bacterial artificial chromosome (BAC) in cells. It contains two integration sites for foreign genes, by Tn7 transposition or, alternatively, by site-specific recombination mediated by the Cre enzyme. MultiBac further consists of an array of plasmids called Acceptors and Donors that facilitate multigene assembly. MultiBac baculovirions ( ) are generated by transfecting composite MultiBac BAC in insect cells. MultiBac is successfully used for a wide variety of applications in basic and applied research and development ( ). Genes of interest are shown as indicate LoxP sites. Origins of replication appear as rectangular shapes. phage-derived replicon, virus-like particle, kanamycin marker, Ampicillin marker, Cre-LoxP fusion reaction, blue/white selection cassette, Tn7 attachment site