Lipoteichoic acid of Enterococcus faecalis inhibitsosteoclastogenesis via transcription factor RBP-J

摘要

Lipoteichoic acid (LTA) of is a potentstimulator of inflammatory responses, but the effects of LTA on osteoclastogenesis remains far from wellunderstood. This study showed that LTAsignificantly inhibited osteoclastogenesis of wild type murine bonemarrow-derived macrophages (BMMs) in the presence of a high dose of RANKL, whilethe inhibition of osteoclastogenesis by LTA wassignificantly removed in BMMs with deficient expression of the transcriptionfactor RBP-J. In addition, a few small osteoclasts were generated in BMMs withonly LTA stimulation, presumably due to theproduction of TNF-α and IL-6. Furthermore, both p38 and ERK1/2 MAPK signalingpathways were activated after 24 h of LTAtreatment, but these signaling pathways were not activated after 6 d oftreatment with RANKL in mature osteoclasts. In conclusion, LTA, which induces inflammatory response, could inhibitRANKL-induced osteoclastogenesis via RBP-J in BMMs.