Simultaneous use of direct and indirect diagnostic techniques in atypical respiratory infections from Chlamydophila pneumoniae and Mycoplasma pneumoniae

摘要

In 2008, 50 samples (BAL), coming from hospital patients, with acute respiratory symptoms have been investigated using two real‐time PCR methods: one assay for the single detection of and DNA and one commercially available real‐time duplex PCR assay for the detection of . and . DNA. Both techniques used here showed compliant results, with 100% concordance for detection of . and 98% for detection of . . The positive results obtained agreed with the clinical suspicion of such infections in some cases and with the presence of IgM specific for . and . in all cases of acute infection. J. Clin. Lab. Anal. 23:206–209, 2009. © 2009 Wiley‐Liss, Inc.