A partial T-DNA insertion near KNAT1 results in lobed Arabidopsisthaliana leaves

摘要

(A) Diagram of the MIGS T-DNA composed of left border (LB), Basta herbicide resistance (BAR) gene, the CaMV 35S promoter (35S), the miR173 target sequence, and the right border (RB). (B) Diagram of the T-DNA insertion directly upstream of ( ) in the Gamora mutant. Location of the 35S For and At4:5152339 Rev primers used for the PCR are shown as black arrows. Note that part of the miR173 target sequence and the entire right border are missing. Gene diagrams were made using Geneious version 2020.0 created by Biomatters. (C) Comparison of wild-type (WT) and the Gamora mutant phenotype (MT) at 6 weeks of development. (D) PCR results from amplification of wild-type (WT) and Gamora mutant (MT) DNA with 35S For and At4:5152339 Rev primers. The 600 bp band in the MT lane indicates that the Gamora mutant contains the T-DNA as shown in B.