A Conserved Carboxylesterase Inhibits Tobacco mosaic virus (TMV) Accumulation in Nicotiana benthamiana Plants

摘要

A carboxylesterase (CXE) or carboxylic-ester hydrolase is an enzyme that catalyzes carboxylic ester and water into alcohol and carboxylate. In plants, CXEs have been implicated in defense, development, and secondary metabolism. We discovered a new CXE gene in that is related to virus resistance. The transcriptional level of NbCXE expression was significantly increased after (TMV) infection. Transient over-expression of NbCXE inhibited TMV accumulation in plants. Conversely, when the NbCXE gene was silenced with a (TRV)-based gene silencing system, TMV RNA accumulation was increased in NbCXE-silenced plants after infection. NbCXE protein was shown to interact with TMV coat protein (CP) in vitro. Additionally, the expressions of host defense-related genes were increased in transient NbCXE-overexpressed plants but decreased in NbCXE silenced plants. In summary, our study showed that NbCXE is a novel resistance-related gene involved in host defense responses against TMV infection.