Akkermansia muciniphila protects intestinal mucosa from damage caused by S. pullorum by initiating proliferation of intestinal epithelium

摘要

Newborn chicks were orally administrated with 200 μL PBS or (10  CFU) suspended in 200 μL PBS once a day, for a period of 10 days. Chicks were orally administered (10  CFU) on the fifth day. On the 10 day, chickens were sacrificed and subsequent experiments were performed. Changes in chicks’ body weight were monitored each day;  = 12. The anatomical morphology of chicks treated with PBS, , or plus . The areas marked with an arrow show where the colon became shorter, thinner and transparent, and the cecum abscess. Photomicrographs of the chick colons. The areas marked by the arrow were villous shedding and colonic epithelial cell damage caused by . Scale bars 200 μm. CMDI score of different groups. and Chicks were orally administered (10  CFU), a standard curve of was established and chicks’ stool DNA was extracted from fresh manure, the number of in chick feces at the indicated time points was detected by quantitative RT-PCR. The expression of IL-1β in the four groups was detected using an ELISA kit;  = 12. The expression of TNF-α in the four groups was detected using an ELISA kit;  = 12.