Dorsal vagal complex and hypothalamic glia differentially respond to leptin and energy balance dysregulation

摘要

Immunohistochemical visualization of Cy5-Lep (4V icv 4.5 ng; red) colocalized to DVC astrocytes (GFAP; green), DAPI in blue; 63x image (inset: 2x optical zoom). FISH visualization of the lateral NTS (at the level of the AP) of LEP-Rb (yellow) expression on DVC astrocytes (ALDH1L1; cyan) and neurons (RbFox3; magenta). Counterstain with DAPI (blue); 40x image (inset: 3.4x optical zoom with gaussian filter). See Supplementary Video for 3D rotational image of area outlined by the dotted box.