Generation of functional salivary gland tissue from human submandibular gland stem/progenitor cells

摘要

Isolation, identification, and characterization of hSMGepiS/PCs. Images of primary cells isolated and passaged in 2D culture. Scale bar = 200 μm. Representative images of single cells 3D-cultured and spheres formed. Scale bar = 1 mm (above) and 200 μm (below). Quantification of sphere formation of hSMGepiS/PCs from different donors at day 7 after 3D culture. Error bars, SD,  = 6. Immunofluorescence of K19 in cells and hSMGs to locate the isolated cells. Scale bar = 100 μm. Positive expression of an epithelial marker, E-cadherin, in isolated cells. Scale bar = 100 μm. Immunofluorescence showing that isolated cells express the proliferation marker, Ki67, and the epithelial stem/progenitor cell markers, K5 and CD49f. Scale bar = 100 μm. Protein levels of the indicated markers in hSMGepiS/PCs and hSMGMCs by western blotting